[PYI-9100:ERROR] Failed to execute script 'MultiProcess' due to unhandled exception

[alice01925a]

Hello,

I am trying to run pLink3 on my timsTOF data set with a DSS crosslinker.

I run into this issue randomly in samples with same analysis method:

[pParse2Plus] "E:\ChiaHsin\1116BSADSS\plinkResult\3407pLink3\3407pLink3.plink" " -t -0.5 -z 5 -i 1 -C 1 -a 0 -p 1 -S 1 -b 1 -m 0 -P 1 -F TIMS -n 1" " -path:pParse2 ..\pXtract3\pParse.exe -Plus:pxtract_n_isotope 0"
[pParse2Plus] 0, cfg_plus_args: {'path': {'pParse2': '..\pXtract3\pParse.exe'}, 'Plus': {'pxtract_n_isotope': 0}}
[pParse2Plus] 0, E:\ChiaHsin\1116BSADSS\3407.d
[cur] 2025-11-19 10:13:59 Starting...
[pParse2Plus] 0, Extract using pParse
[pParse2Plus] "C:\pFindStudio\pLink\3.0.17\bin\pXtract3\pParse.exe" -D "E:\ChiaHsin\1116BSADSS\3407.d" -t -0.5 -z 5 -i 1 -C 1 -a 0 -p 1 -S 1 -b 1 -m 0 -P 1 -F TIMS -n 1 -O "E:\ChiaHsin\1116BSADSS"

  *********************************************
 /       pParse2.0 (x64) from pFind Studio     \
/        Email  :  pfind@ict.ac.cn              \
\        Website:  http://pfind.org             /
  *********************************************

[pParse] E:\ChiaHsin\1116BSADSS\3407.d Converting to MS1 & MS2...
[pXtract] The software will expire in 01/01/2100
[pXtract] Begin to extract E:\ChiaHsin\1116BSADSS\3407.d
[pXtract] Acquisition mode: DDA
[pXtract] Frame: 33778 Precursor: 71329 MSMS: 197871
[pXtract] : 0.00%
[pParse] == == == Time elapsed: 3 seconds. == == == =
[cur] 2025-11-19 10:14:03 Using: 0hr0min4s, 0, End pParse2Plus!
[MultiProcess] "E:\ChiaHsin\1116BSADSS\plinkResult\3407pLink3\3407pLink3.plink"
[MultiProcess] Start...
[ERROR] Traceback (most recent call last):
File "MultiProcess.py", line 78, in
File "MultiProcess.py", line 70, in _main
File "mp_flow.py", line 623, in mp_flow.flow_auto
File "mp_flow.py", line 65, in mp_flow.sort_raw
File "mp_flow.py", line 39, in mp_flow.stat_pf2_spec_num
File "mp_flow.py", line 40, in mp_flow.stat_pf2_spec_num
struct.error: unpack requires a buffer of 8 bytes
[PYI-9100:ERROR] Failed to execute script 'MultiProcess' due to unhandled exception!

Is there a reason why the analysis is not working?
Thank you