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@phlaster phlaster commented Jul 17, 2025

I was constructing primers for nested PCR recently and found your package, which came in really handy. However, for the output primers, I couldn't obtain their exact amplicon coordinates (alignment-wise), which is crucial because, in nested PCR, the inner amplicon should be inside the outer one. Therefore, I decided to contribute a small modification that adds two columns to the output CSV, indicating the 1-based starting and ending positions of each amplicon.

@phlaster phlaster changed the title Start and stop positions of amplicons in csv output Start and stop amplicon positions in csv output Jul 18, 2025
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